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BISC110/F12: Series 3 Experiment 9 Hill Reaction: Difference between revisions
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BISC110/F12: Series 3 Experiment 9 Hill Reaction (view source)
Revision as of 10:49, 10 May 2012
, 10 May 2012→Procedure
Tucker Crum (talk | contribs) |
Tucker Crum (talk | contribs) |
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#Be sure that your blank (thylakoids in resuspension solution) is loaded in a 13mm test tube, not a plastic cuvette. | #Be sure that your blank (thylakoids in resuspension solution) is loaded in a 13mm test tube, not a plastic cuvette. | ||
#Wipe outside of test tube with a Kimwipe, insert it into the Tube Holder (C), and close the lid. | #Wipe outside of test tube with a Kimwipe, insert it into the Tube Holder (C), and close the lid. | ||
#Use 100% Adjust Knob (B) to set meter to 100%T, which is equivalent to zero absorbance on the absorbance scale. Remove the blank tube and save it in your ice bucket | #Use 100% Adjust Knob (B) to set meter to 100%T, which is equivalent to zero absorbance on the absorbance scale. Remove the blank tube and save it in your ice bucket. | ||
<br><br> | <br><br> | ||
'''Varying the Reaction Rate:''' The Hill reaction occurs at different rates depending on the light intensity supplied to the thylakoids. Thylakoid concentration is also an important variable. Our goal today is to determine the best light intensity and thylakoid concentration for a 90 sec. reaction. You will measure the reaction rates by taking absorbance readings over that time period in 15 s intervals. We will use the drop in absorbance over time as a measure of reduction of blue DCPIP to colorless DCPIPH. If we can control all the variables properly, this reduction rate is also a measurement of the rate of photosynthesis. <br><br> | '''Varying the Reaction Rate:''' The Hill reaction occurs at different rates depending on the light intensity supplied to the thylakoids. Thylakoid concentration is also an important variable. Our goal today is to determine the best light intensity and thylakoid concentration for a 90 sec. reaction. You will measure the reaction rates by taking absorbance readings over that time period in 15 s intervals. We will use the drop in absorbance over time as a measure of reduction of blue DCPIP to colorless DCPIPH. If we can control all the variables properly, this reduction rate is also a measurement of the rate of photosynthesis. <br><br> | ||
