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BISC110/F12: Series 3 Experiment 9 Hill Reaction: Difference between revisions
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BISC110/F12: Series 3 Experiment 9 Hill Reaction (view source)
Revision as of 06:38, 10 May 2012
, 10 May 2012→Procedure
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In this lab you will examine the light reactions of photosynthesis by measuring the so-called Hill reaction in lysed chloroplasts. You will practice these measurements until you get reproducible results. Next week you will investigate the effect of various environmental factors on the light reactions of photosynthesis. <br> | In this lab you will examine the light reactions of photosynthesis by measuring the so-called Hill reaction in lysed chloroplasts. You will practice these measurements until you get reproducible results. Next week you will investigate the effect of various environmental factors on the light reactions of photosynthesis. <br> | ||
Be sure to keep all beakers and buffers on ice. Note that there are four different media used: grinding medium (100 mM Tricine NaOH pH 7.8, 400 mM sorbitol, 5 mM MgCl2), breaking medium (20 mM Tricine NaOH pH 7.8, 5 mM MgCl<sub>2</sub>), resuspension medium (50 mM , 100 mM sorbitol, 5 mM MgCl<sub>2</sub>)and reaction solution (100 mM sorbitol, 5 mM MgCl<sub>2</sub>, 50 mM NaPO<sub>4</sub>, 0.05 mM DCPIP). Note the differences among them. Be sure to use the correct solution. Read labels carefully throughout the experiment. | Be sure to keep all beakers and buffers on ice. Note that there are four different media used to prepare a thylakoid suspension: grinding medium (100 mM Tricine NaOH pH 7.8, 400 mM sorbitol, 5 mM MgCl2), breaking medium (20 mM Tricine NaOH pH 7.8, 5 mM MgCl<sub>2</sub>), resuspension medium (50 mM , 100 mM sorbitol, 5 mM MgCl<sub>2</sub>)and reaction solution (100 mM sorbitol, 5 mM MgCl<sub>2</sub>, 50 mM NaPO<sub>4</sub>, 0.05 mM DCPIP). Note the differences among them. Be sure to use the correct solution. Read labels carefully throughout the experiment. | ||
<br><br> | <br><br> | ||
'''Preparation of thylakoid stock solution''' | '''Preparation of thylakoid stock solution''' | ||
