CH391L/S12/Selectablegeneticmarkers: Difference between revisions

From OpenWetWare
Jump to navigationJump to search

CH391L/S12/Selectablegeneticmarkers (view source)

Revision as of 23:20, 26 February 2012

637 bytes added ,  26 February 2012
no edit summary
No edit summary
No edit summary
 
Line 28: Line 28:
Selectable genetic markers for plants are not always in the form of herbicide resistance. For instance, researchers at China's Agricultural University were able to express the rstB gene in tobacco, which confers upon the plant greater tolerance to salt concentration. <i>Zhang et al.</i> was able to achieve approximately 80% selection efficiency using salt concentrations at 170mM, proving remarkable success in using a selectable genetic marker other than herbicide resistance<cite>Zhang2008</cite>.
Selectable genetic markers for plants are not always in the form of herbicide resistance. For instance, researchers at China's Agricultural University were able to express the rstB gene in tobacco, which confers upon the plant greater tolerance to salt concentration. <i>Zhang et al.</i> was able to achieve approximately 80% selection efficiency using salt concentrations at 170mM, proving remarkable success in using a selectable genetic marker other than herbicide resistance<cite>Zhang2008</cite>.


===TO ADD: Auxotrophic===
===Auxotrophic===


Auxotrophy is an affliction in which an organism cannot produce the compounds necessary for its survival. For instance, humans cannot naturally produce vitamins, and must obtain them from their diet. Auxotrophy can occur in nature due to unfavorable mutations, and almost always leads to an organism's premature death. Auxotrophy can be applied in a laboratory setting by altering an organism so as to remove its ability to produce a necessary compound. For instance, one could employ PCR mediated gene disruption to insert a gene in such a way as to inhibit another gene from functioning. URAFor a list of common auxotrophic selective markers used in yeast, [http://www.yeastgenome.org/community/alleletable.shtml look here]
Auxotrophy is a condition in which an organism cannot produce the compounds necessary for its survival. For instance, humans cannot naturally produce vitamins, and must obtain them from their diet. Auxotrophy can be applied in a laboratory setting by altering an organism so as to remove its ability to produce a necessary compound. For instance, one could employ PCR mediated gene disruption to insert a gene in such a way as to inhibit a gene, crucial for the production of organic compounds, from functioning. This can create a strain of bacteria, yeast, or other easily manipulable organism that is incapable of producing the basic compounds for its survival. However, when supplied with these compounds from their environment, these organisms maintain the ability to survive. This is useful as it creates organisms that will die of their own accord, unless specifically kept alive by experimenters. One can then transform a plasmid into these organisms with a gene that allows for the production of this compound, and grow the organism in media without this compound. Only successfully transformed organisms will survive in this environment.
 
Auxotrophic selective markers are commonly used in experiments involving yeast strains. For a list of common auxotrophic selective markers used in yeast, [http://www.yeastgenome.org/community/alleletable.shtml look here].


===Other===
===Other===
Peter Otoupal
146

edits

Retrieved from "https://openwetware.org/wiki/Special:MobileDiff/587751"

Navigation menu