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In addition to selectable genetic markers are screenable genetic markers. Screenable genetic markers function in a similar manner in that they are extraneous genes that are transformed into a cell; however, they do not confer any new sort of resistance to the cell. Instead, they cause the cell to respond differently to environmental conditions in such a way as to distinguish transformed cells from untransformed cells. This can be useful when determining the transformation efficiency of a cell, or when carefully monitoring the activity of proteins. | In addition to selectable genetic markers are screenable genetic markers. Screenable genetic markers function in a similar manner in that they are extraneous genes that are transformed into a cell; however, they do not confer any new sort of resistance to the cell. Instead, they cause the cell to respond differently to environmental conditions in such a way as to distinguish transformed cells from untransformed cells. This can be useful when determining the transformation efficiency of a cell, or when carefully monitoring the activity of proteins. | ||
==Types of Selectable Markers== | ==Types of Selectable Markers== | ||
==Screening== | ===Antibiotic=== | ||
=== | ==Types of Screening== | ||
===Blue/White=== | |||
[[Image:Blue white test.jpg|thumb|right|Successful example of a blue/white screen test. Blue colonies are wild-type cells, while white colonies are successfully transformed cells]] | |||
===Green Fluorescent Protein=== | ===Green Fluorescent Protein=== | ||
[[Image:Green_Fluroescent_Mice.jpg|thumb|left|Mice transfected with GFP. One can easily distinguish the wild-type mouse (middle) from the two mice with GFP (left and right)<cite>Moen2011</cite>.]] | [[Image:Green_Fluroescent_Mice.jpg|thumb|left|Mice transfected with GFP. One can easily distinguish the wild-type mouse (middle) from the two mice with GFP (left and right)<cite>Moen2011</cite>.]] | ||
