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BioBuilding: Synthetic Biology for Teachers: Lab 1: Difference between revisions
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BioBuilding: Synthetic Biology for Teachers: Lab 1 (view source)
Revision as of 13:00, 9 January 2012
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==Needed Materials== | ==Needed Materials== | ||
===Teacher Provides=== | ===Teacher Provides=== | ||
* | *Sterile toothpicks (or innoculating loop and bunsen burner) | ||
*Sterile tubes for growing liquid cultures of cells | *Sterile tubes for growing liquid cultures of cells, (4) if testing all 4 strains | ||
*Cuvettes to measure absorbances if spectrophotometer is not fitted for glass tubes | *Cuvettes to measure absorbances if spectrophotometer is not fitted for glass tubes | ||
* | *125 ml erlenmeyer flasks + stir bars, (4) if testing all 4 strains | ||
*Stir plates | *Stir plates, (4) if testing all 4 strains | ||
*Pipetmen and tips (P1000, P200, P20) | *Pipetmen and tips (P1000, P200, P20) | ||
*Pipets ( | *Pipets (5 ml) and bulbs | ||
*Timers | *Timers | ||
*Sharpies | *Sharpies | ||
*Nitrile or Latex gloves | *Nitrile or Latex gloves | ||
* | *Roller wheel at room temp or 37° for growing overnight cultures of bacteria (can use platform shaker instead) | ||
*If available, vortex for mixing cells prior to additions | *If available, vortex for mixing cells prior to additions | ||
*If available, fume hood for measuring isoamyl alcohol (aka isopentyl alcohol) | *If available, fume hood for measuring isoamyl alcohol (aka isopentyl alcohol) | ||
===Kit Provides=== | ===Kit Provides=== | ||
====4 strains (see table below)==== | ====4 strains (see table below)==== | ||
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[[Image:Lab2 Kit.jpg|thumb|left|400px]] | [[Image:Lab2 Kit.jpg|thumb|left|400px]] | ||
=====Room Temperature===== | =====Room Temperature===== | ||
*Concentrated Banana Extract to make Banana smell standards | |||
*Concentrated Banana | |||
=====4° (fridge)===== | =====4° (fridge)===== | ||
* | *Ampicillin (100 mg). Can be added to 1 ml in H2O, filter sterilized and used at a 1:1000 dilution in LB. Alternatively, can add entire aliquot to 1 liter of LB and store LB + amp in the fridge. | ||
=====Chemical Hood===== | =====Chemical Hood===== | ||
* 700 ul isoamyl alcohol, enough to prepare 1L of media for lab | * 700 ul isoamyl alcohol, enough to prepare 1L of media for lab | ||
