Neomycin: Difference between revisions

Neomycin (view source)

17 bytes added , 4 August 2006

neo - beta 3 - reformating: too many small headings

2,879

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 ==Experimental==
-===Target organism===
+'''target organism''': bacteria, mammalian cells
-bacteria, mammalian cells
+'''stock solution''': 10mg/ml in water
+'''working solution''': 10-50µg/ml (stable at RT for 5days)
-===Stock solution===
-mg/ml in water
-===Working solution===
--50µg/ml
 ==Description==
-===Mode of action===
+'''mode of action''': binds to ribosomal components and inhibits protein synthesis.
-Binds to ribosomal components and inhibits protein synthesis.
+'''mode of resistance''': ''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation.
-===Mode of resistance===
-''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation.
-==Related antibiotics==
+==Links==
-[[kanamycin]], [[G418]]
-==External links==
+* related antibiotics: [[kanamycin]], [[G418]]
 * list of cell culture reagents by Krackeler [http://www.krackeler.com/products/fid/2910]
 * list of cell culture reagents by USB [http://www.usbweb.com/reference2.asp?id_ref=14]
 * forum thread on difference between neomycin & G418 [http://www.protocol-online.org/forums/index.php?showtopic=10608&hl=neomycin]