Choosing reference genes for qPCR normalisation: Difference between revisions

:All genes used for normalization can show problems in one or the other condition. There are always conditions in which their expression differs significantly from their general level of expression. That is why reference genes should be validated for one's condition of interest. Ideally, one should choose from the complete genome rather than from a gene panel. Genevestigator RefGenes [http://www.refgenes.org] is a publicly available online tool that uses a very large microarray database to identify genes that are most stable in conditions similar to that of your own experiment.