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McClean: Tetrad Dissection: Difference between revisions
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McClean: Tetrad Dissection (view source)
Revision as of 13:22, 30 September 2011
, 30 September 2011→Dissection
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## Pick up the four spores with the microneedle. I like to place the needle very near to (but not touching) the spores, and if they are a genuine tetrad they will be sucked by surface tension as a group toward the needle. | ## Pick up the four spores with the microneedle. I like to place the needle very near to (but not touching) the spores, and if they are a genuine tetrad they will be sucked by surface tension as a group toward the needle. | ||
## Place them on the agar in a click position at least 5 mm from the stripe of b-glucoronidase-treated cells. Note the position on the mechanical stage. | ## Place them on the agar in a click position at least 5 mm from the stripe of b-glucoronidase-treated cells. Note the position on the mechanical stage. | ||
*# To break apart the four spores, place the needle so that it barely touches the tetrad then gently tap the base of the microscope or table beside the microscope. This vibrates the needle enough to break apart the tetrad without jabbing the needle into the agar. | |||
## Pick up three spores. Move the stage 5 mm (one click) further away from the streak and deposit the three spores. | ## Pick up three spores. Move the stage 5 mm (one click) further away from the streak and deposit the three spores. | ||
## Pick up two spores. Move the stage 5 mm (one click) away from the streak and deposit the two spores. | ## Pick up two spores. Move the stage 5 mm (one click) away from the streak and deposit the two spores. | ||
