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BioBuilding: Synthetic Biology for Teachers: Lab 1: Difference between revisions
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BioBuilding: Synthetic Biology for Teachers: Lab 1 (view source)
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This lab offers two different protocols based on the time the teacher wishes to allow. Each of these protocols covers the same concepts but allows for different emphases. | This lab offers two different protocols based on the time the teacher wishes to allow. Each of these protocols covers the same concepts but allows for different emphases. | ||
[[BioBuilding:_Synthetic_Biology_for_Teachers:_Lab_1--Protocol_A_(Condensed) |Protocol A--Condensed data collection]]: This is a shorter procedure for the students. This shorter protocol allows for more | [[BioBuilding:_Synthetic_Biology_for_Teachers:_Lab_1--Protocol_A_(Condensed) |Protocol A--Condensed data collection]]: This is a shorter procedure for the students. This shorter protocol allows for more data analysis but less data collection. With this protocol the teacher can choose to have the students do the initial bacterial culturing if more microbial techniques are to be emphasized. Essentially, a day prior to any data collection, the large cultures are set up. Part of the starter culture is immediately removed and placed in the refrigerator. This serves as the lag phase sample. After 5-7 hours, a second sample is removed. This serves as the log phase sample. The last third of the culture should be allowed to grow overnight. This serves as the stationary phase sample. The samples can then be provided to the students the following class day, allowing the students to collect data in single lab period. Go to [[BioBuilding:_Synthetic_Biology_for_Teachers:_Lab_1--Protocol_A_(Condensed) |Protocol A]] for the detailed protocol. | ||
[[BioBuilding:_Synthetic_Biology_for_Teachers:_Lab_1--Protocol_B_(expanded) |Protocol B--Growth curve data collection]]: This version provides a greater emphasis on collection of growth curve data. In this version, the students will create stocks from the overnight cultures and sample the banana smell and turbidity (population) every twenty minutes. If possible, different classes can measure the same stocks to increase the number of data points. At the end of the first day data collection, stocks can be refrigerated and re-incubation can begin about 30 minutes prior to the next data collection. You should expect to collect data over three days to get multiple data points from all phases of the growth curve. Go to [[BioBuilding:_Synthetic_Biology_for_Teachers:_Lab_1--Protocol_B_(expanded) |Protocol B]] for the detailed protocol. | [[BioBuilding:_Synthetic_Biology_for_Teachers:_Lab_1--Protocol_B_(expanded) |Protocol B--Growth curve data collection]]: This version provides a greater emphasis on collection of growth curve data. In this version, the students will create stocks from the overnight cultures and sample the banana smell and turbidity (population) every twenty minutes. If possible, different classes can measure the same stocks to increase the number of data points. At the end of the first day data collection, stocks can be refrigerated and re-incubation can begin about 30 minutes prior to the next data collection. You should expect to collect data over three days to get multiple data points from all phases of the growth curve. Go to [[BioBuilding:_Synthetic_Biology_for_Teachers:_Lab_1--Protocol_B_(expanded) |Protocol B]] for the detailed protocol. | ||
