BioBuilding: Synthetic Biology for Teachers: Lab 1: Difference between revisions

[[BioBuilding:_Synthetic_Biology_for_Teachers:_Lab_1--Protocol_A_%28Condensed%29&action=edit&redlink=1 |Protocol A--Condensed data collection]]: This is a shorter procedure for the students. This shorter protocol allows for more emphasis on microbiological techniques. This protocol is recommended should the teacher choose to have the students do the initial bacterial culturing. Essentially, a day prior to any data collection, the large cultures are set up. Instead of letting them all run, part of the culture is immediately removed and placed in the refrigerator. This will be the lag phase sample. After 5-7 hours, a second sample is removed. This will be the log phase sample. The last third of the culture will be allowed to grow overnight. This will be the stationary phase sample. These samples can then be provided to the students the next day. This will allow the students to easily obtain data in one lab period. Go to [[BioBuilding:_Synthetic_Biology_for_Teachers:_Lab_1--Protocol_A_%28Condensed%29&action=edit&redlink=1 |Protocol A]] for the detailed protocol.

[[BioBuilding:_Synthetic_Biology_for_Teachers:_Lab_1--Protocol_B_%28expanded%29&action=edit&redlink=1 |Protocol B--Growth curve data collection]]: This version provides a greater emphasis on collection of growth curve data. In this version, the students will create stocks from the overnight cultures and sample the banana smell and turbidity (population) every twenty minutes. If possible, different classes can measure the same stocks to increase the number of data points. At the end of the first day data collection, stocks can be refrigerated and re-incubation can begin about 30 minutes prior to the next data collection. You should expect to collect data over three days to get multiple data points from all phases of the growth curve. Go to [[BioBuilding:_Synthetic_Biology_for_Teachers:_Lab_1--Protocol_B_%28expanded%29&action=edit&redlink=1 |Protocol B--Growth curve data collection]] for the detailed protocol.