IPTG: Difference between revisions

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Generally a 1mM solution is an effective amount to induce the pLac promoter region.  It should be noted that this may vary over cell strains.  For example:  
Generally a 1mM solution is an effective amount to induce the pLac promoter region.  It should be noted that this may vary over cell strains.  For example:  
*lacIq: cell strains which overproduce lacI repressor (ie. [[E. coli genotypes|E.coli cell strain]] type [[http://openwetware.org/wiki/E._coli_genotypes#D1210|D1210]], aka. [http://parts.mit.edu/registry/index.php/Part:BBa_V1003 BBa_V1003])
*lacIq: cell strains which overproduce lacI repressor (ie. [[E. coli genotypes|E.coli cell strain]] type [[E. coli genotypes#D1210|D1210]], aka. [[registry:Part:BBa_V1003|BBa_V1003]])
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