Griffin:siRNA transfection: Difference between revisions

Griffin:siRNA transfection (view source)

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Revision as of 09:37, 4 May 2011 (view source) Korey Griffin (talk \| contribs) (→‎Suspension Cells) ← Older edit		Revision as of 09:37, 4 May 2011 (view source) Korey Griffin (talk \| contribs) (→‎Sorting Dead Suspension Cells=) Newer edit →
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	====Sorting Dead Suspension Cells=====		====Sorting Dead Suspension Cells====

	Dead Cells are generally lighter than live cells. Resuspend the cells in 15 or 50 ml conical centrifuge tubes and allow the cells to settle 2-5 minutes. Most live cells will sediment to the bottom, leaving a mixture of Live/Dead cells floating in the supernatant. Aspirate supernatant and discard. This will leave the live cells ready for resuspension into the culture flask. Some live cells will be lost in this process.		Dead Cells are generally lighter than live cells. Resuspend the cells in 15 or 50 ml conical centrifuge tubes and allow the cells to settle 2-5 minutes. Most live cells will sediment to the bottom, leaving a mixture of Live/Dead cells floating in the supernatant. Aspirate supernatant and discard. This will leave the live cells ready for resuspension into the culture flask. Some live cells will be lost in this process.