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Griffin:siRNA transfection: Difference between revisions
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==Design | ==Design== | ||
The methodology for designing siRNA is well established in primary literature from ~2002-2005; thermodynamics, filters, motif parameters. Rational design is based on the substrate characteristics of miRNA, however does not predict steric effects for antisense recognition of the transcript, hence the attractiveness of pools. | |||
===siRNA Design Scoring=== | ===siRNA Design Scoring=== | ||
