2,332
edits
Griffin:Antibody Basics: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Rodent (mouse, rat, hamster)
| Line 136: | Line 136: | ||
Small mammal that requires less antigen than the rabbit or goat. Suitable for spleenectomy and B cell fusion to produce monoclonal Ig. | Small mammal that requires less antigen than the rabbit or goat. Suitable for spleenectomy and B cell fusion to produce monoclonal Ig. | ||
mice spleen is isolated and fused to myeloma to produce immortal Ig producing B-Cells secreting Ig into the culture supernatant. Each subcloned 'single' hybridoma B cell produces a 'mono' single clone Ig that recognizes only 1 epitope region. Large antigens are suitable to immunize mice, however the monoclonal Ig will only target a single epitope within the antigenic region, as small as 3 amino acids. | |||
===Chicken=== | ===Chicken=== | ||
