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Griffin:siRNA transfection: Difference between revisions
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→Working Cell Density Parameters
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==Working Cell Density Parameters== | ==Working Cell Density Parameters== | ||
===Suspension Cells=== | |||
Suspension cells require slow speed centrifugation (2500xg) to collect intact cell pellets from cuture flasks, then counting the cells with a hemocytometer and then seeding the wells accordingly by cell count into transfection medium. Suspension cells can proceed directly to the siRNA experiment rather than adherent cells, that require seeding the cells and waiting for adherence. | |||
===Six well plates (2x10e5 cells per well)=== | ===Six well plates (2x10e5 cells per well)=== | ||
