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Beta-galactosidase Screen: Difference between revisions
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→Agar Overlay
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#Microwave 10ml of water with 0.15g agar for each plate to be screened (e.g. for 10 plates it's 100ml water and 1.5g agar). | #Microwave 10ml of water with 0.15g agar for each plate to be screened (e.g. for 10 plates it's 100ml water and 1.5g agar). | ||
#Let the molten agar cool in a 60°C water bath. | #Let the molten agar cool in a 60°C water bath. | ||
#Add 2μL X-gal stock for each mL of molten agar (e.g. 200μL for | #Add 2μL X-gal stock for each mL of molten agar (e.g. 200μL for 10 plates). | ||
#Pour 10 ml over each plate to be assayed. | #Pour 10 ml over each plate to be assayed. | ||
#Let solidify | #Let solidify | ||
