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Beta-galactosidase Screen: Difference between revisions
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→Agar Overlay
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===Agar Overlay=== | ===Agar Overlay=== | ||
*This method is used if: your media has a non-neutral pH, you forgot to do one of the other two protocols, or some undergrad in your lab mislabeled the plates. | *This method is used if: your media has a non-neutral pH, you forgot to do one of the other two protocols, or some undergrad in your lab mislabeled the plates. | ||
*Color should appear on fully developed colonies | *Color should appear on fully developed colonies in 2-8 hours. | ||
#Microwave 10ml of water with 0.15g agar for each plate to be screened (e.g. for 10 plates its 100ml water and 1.5g agar). | #Microwave 10ml of water with 0.15g agar for each plate to be screened (e.g. for 10 plates its 100ml water and 1.5g agar). | ||
#Let the molten agar cool in a 60°C water bath. | #Let the molten agar cool in a 60°C water bath. | ||
