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Beta-galactosidase Screen: Difference between revisions
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→Spread onto LB plates
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#Once solid keep out of light. | #Once solid keep out of light. | ||
===Spread onto | ===Spread onto plates=== | ||
*This method is used to turn any kind of plate into an X-gal plate. The media should have a neutral pH. | *This method is used to turn any kind of plate into an X-gal plate. The media should have a neutral pH. | ||
*Colonies should develop color overnight, but due to uneven distribution of X-gal, all positive colonies may not be evenly colored. | *Colonies should develop color overnight, but due to uneven distribution of X-gal, all positive colonies may not be evenly colored. | ||
