Richard Lab:Amplified insert assembly: Difference between revisions

Richard Lab:Amplified insert assembly (view source)

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 ==Overview==
-Amplified Insert Assembly is a method of "BioBricking" two biological parts (i.e. pieces of DNA) together and was developed by [[User:Michael A. Speer|Mike Speer]] and Dr. Tom Richard.  For more information on bio-bricking see [http://partsregistry.org/Help:Contents this link].  This method combines the ease and speed of 3A assembly with the reliability of standard assembly.  In comparison to 3A assembly, this method can take up to two hours longer; however, the added time spent at the bench is minimal.  Major benefits of this assembly method over other bio-brick assembly methods include:
+Amplified Insert Assembly is a method of "BioBricking" two biological parts (i.e. pieces of DNA) together and was developed by [[User:Michael A. Speer|Mike Speer]] and Dr. Tom Richard.  For more information on bio-bricking see [http://partsregistry.org/Help:Contents this link].  This method combines the ease and speed of 3A assembly with the reliability of standard assembly.  In comparison to [[Synthetic Biology:BioBricks/3A assembly|3A assembly]], this method can take up to two hours longer; however, the added time spent at the bench is minimal.  Major benefits of this assembly method over other bio-brick assembly methods include:
 *no need for [[Richard Lab:Agarose Gel Electrophoresis|gel electrophoresis]] or [[DNA Gel extraction|gel extraction]].