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Richard Lab:Amplified insert assembly: Difference between revisions
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Richard Lab:Amplified insert assembly (view source)
Revision as of 17:00, 25 January 2011
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*The "insert" will only be digested for one hour. This is okay as there is a lot of it. | *The "insert" will only be digested for one hour. This is okay as there is a lot of it. | ||
*Detractors of this method may say that it's risky to PCR the inserts because of mutations. We say: | *Detractors of this method may say that it's risky to PCR the inserts because of mutations. We say: | ||
::#This hasn't been a problem for us. | |||
;;#This is why we use a high-fidelity polymerase | |||
::#We're sequencing the constructs anyway so we'd spot any mutations. | |||
==Contact== | ==Contact== | ||
