1,721
edits
Richard Lab:Amplified insert assembly: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Richard Lab:Amplified insert assembly (view source)
Revision as of 15:22, 24 January 2011
, 24 January 2011→Overview
Kay Dimarco (talk | contribs) |
|||
| Line 14: | Line 14: | ||
<center>'''-----EcoRI--XbaI--Part--SpeI--PstI-----'''</center> | <center>'''-----EcoRI--XbaI--Part--SpeI--PstI-----'''</center> | ||
Ocassionally other enzymes (e.g. BamHI or HindIII) are used to make protein fusions. | Ocassionally other enzymes (e.g. BamHI or HindIII) are used to make protein fusions. See our [[Richard Lab:Bio-Brick Formats|bio-brick format page]] for more details. | ||
The two parts you want to assemble will be labeled "insert" and "vector" and will be initially contained on separate plasmids. The eventual goal of assembly is to get these parts on the same plasmid next to one another. | The two parts you want to assemble will be labeled "insert" and "vector" and will be initially contained on separate plasmids. The eventual goal of assembly is to get these parts on the same plasmid next to one another. | ||
