2,216
edits
BISC110/S11: Lab 6 Taster SNP1: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
BISC110/S11: Lab 6 Taster SNP1 (view source)
Revision as of 09:27, 7 December 2010
, 7 December 2010→Lab 6 - Taster SNP Week 1
| Line 86: | Line 86: | ||
'''PART III: PCR of your PTC gene (using PuRe Taq Ready-To-Go Master Mix beads ( | '''PART III: PCR of your PTC gene (using PuRe Taq Ready-To-Go Master Mix beads ([http://www.gelifesciences.com GE Healthcare]) '''<BR> | ||
Next, you will set up a polymerase chain reaction (PCR) designed to amplify from your extracted DNA the region of the PTC gene which contains the middle SNP (the one which creates a Fnu4H1 site in the taster allele). We need to use PCR so that we will have enough sample to allow us to visualize the DNA on a gel. The forward and reverse priming sites for our reactions are shown in Figure 1. In your notebook record the sequences of the forward and reverse primers. Use the convention of writing the primer sequence beginning with the 5’ end. '''Have your instructor check your sequences before proceeding.'''<br><br> | Next, you will set up a polymerase chain reaction (PCR) designed to amplify from your extracted DNA the region of the PTC gene which contains the middle SNP (the one which creates a Fnu4H1 site in the taster allele). We need to use PCR so that we will have enough sample to allow us to visualize the DNA on a gel. The forward and reverse priming sites for our reactions are shown in Figure 1. In your notebook record the sequences of the forward and reverse primers. Use the convention of writing the primer sequence beginning with the 5’ end. '''Have your instructor check your sequences before proceeding.'''<br><br> | ||
