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Eccles:QPCR reference genes: Difference between revisions
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[[Eccles Lab]] > [[Eccles:DGG_oligos | DGG Oligos]] > [[Eccles:QPCR_oligos | QPCR]] > reference genes | [[Eccles Lab]] > [[Eccles:DGG_oligos | DGG Oligos]] > [[Eccles:QPCR_oligos | QPCR]] > reference genes | ||
==Human== | |||
*RTPrimerDB ID: 2219 | |||
ACTB: actin, beta | |||
Organism: Homo sapiens (Hs, Human) | |||
Forward Primer: TCCCTGGAGAAGAGCTACG (19 bp) | |||
Reverse Primer: GTAGTTTCGTGGATGCCACA (20 bp) | |||
Annealing Temperature: 60 °C | |||
131 bp | |||
*RTPrimerDB ID: 5 | |||
Gene Information | |||
HPRT1: hypoxanthine phosphoribosyltransferase 1 (Lesch-Nyhan syndrome) | |||
Alias Symbol(s): HPRT, HGPRT | |||
Organism: Homo sapiens (Hs, Human) | |||
(length: 94 bp) | |||
Genomic Amplicon: 4893bp | |||
Forward Primer: TGACACTGGCAAAACAATGCA (21 bp) Exon 6 | |||
Reverse Primer: GGTCCTTTTCACCAGCAAGCT (21 bp) Exon 6/7 | |||
Annealing Temperature: 60 °C | |||
*RTPrimerDB ID: 8 | |||
Gene Information | |||
UBC: ubiquitin C | |||
Alias Symbol(s): HMG20 | |||
Organism: Homo sapiens (Hs, Human) | |||
(length: 133 bp) | |||
Forward Primer: ATTTGGGTCGCGGTTCTTG (19 bp) | |||
Reverse Primer: TGCCTTGACATTCTCGATGGT (21 bp) | |||
Annealing Temperature: 60 °C | |||
*RTPrimerDB ID: 9 | |||
Gene Information | |||
YWHAZ: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide | |||
Alias Symbol(s): KCIP-1 | |||
Organism: Homo sapiens (Hs, Human) | |||
(length: 94 bp) | |||
Forward Primer: ACTTTTGGTACATTGTGGCTTCAA (24 bp) | |||
Reverse Primer: CCGCCAGGACAAACCAGTAT (20 bp) | |||
Annealing Temperature: 60 °C | |||
*RTPrimerDB ID: 4 | |||
Gene Information | |||
HMBS: hydroxymethylbilane synthase | |||
Alias Symbol(s): AIP, UPS, PBGD | |||
Organism: Homo sapiens (Hs, Human) | |||
This primer pair amplifies part of the following transcript: | |||
ENST00000278715 (Exons: 14, Transcript length: 1501 bp, Translation length: 361 residues) | |||
(length: 64 bp) | |||
Genomic amplicon:3252bp | |||
Forward Primer: GGCAATGCGGCTGCAA (16 bp) Exon 1 | |||
Reverse Primer: GGGTACCCACGCGAATCAC (19 bp) Exon 2 | |||
Annealing Temperature: 60 °C | |||
*RTPrimerDB ID: 6 | |||
Gene Information | |||
RPL13A: ribosomal protein L13a | |||
Organism: Homo sapiens (Hs, Human) | |||
This primer pair amplifies part of the following transcript: | |||
ENST00000270634 (Exons: 8, Transcript length: 1125 bp, Translation length: 203 residues) | |||
(length: 126 bp) | |||
Forward Primer: CCTGGAGGAGAAGAGGAAAGAGA (23 bp) | |||
Reverse Primer: TTGAGGACCTCTGTGTATTTGTCAA (25 bp) | |||
Annealing Temperature: 60 °C | |||
*RTPrimerDB ID: 7 | |||
Gene Information | |||
SDHA: succinate dehydrogenase complex, subunit A, flavoprotein (Fp) | |||
Alias Symbol(s): FP, SDH2, SDHF | |||
Organism: Homo sapiens (Hs, Human) | |||
This primer pair amplifies part of the following transcripts: | |||
ENST00000264932 (Exons: 15, Transcript length: 2280 bp, Translation length: 664 residues) | |||
(length: 86 bp) | |||
ENST00000327872 (Exons: 13, Transcript length: 1880 bp, Translation length: 517 residues) | |||
(length: 86 bp) | |||
Forward Primer: TGGGAACAAGAGGGCATCTG (20 bp) | |||
Reverse Primer: CCACCACTGCATCAAATTCATG (22 bp) | |||
Annealing Temperature: 60 °C | |||
*RTPrimerDB ID: 2627 | |||
Gene Information | |||
TBP: TATA box binding protein | |||
Alias Symbol(s): GTF2D, SCA17, TFIID, GTF2D1 | |||
Organism: Homo sapiens (Hs, Human) | |||
This primer pair amplifies part of the following transcript: | |||
ENST00000230354 (Exons: 8, Transcript length: 1845 bp, Translation length: 339 residues) | |||
(length: 132 bp) | |||
Forward Primer: TGCACAGGAGCCAAGAGTGAA (21 bp) | |||
Reverse Primer: CACATCACAGCTCCCCACCA (20 bp) | |||
Annealing Temperature: 60 °C | |||
*GAPDH | |||
*B2M | |||
*28S | |||
28S, from qpcrlist message 3097 <http://groups.yahoo.com/group/qpcrlistserver/message/3097> | |||
Multi species | |||
F tcatcagaccccagaaaagg | |||
R gattcggcaggtgagttgtt | |||
*PPIB | |||
