Eccles:QPCR reference genes

Eccles Lab > DGG Oligos > QPCR > reference genes

Human

• RTPrimerDB ID: 2219

ACTB: actin, beta Organism: Homo sapiens (Hs, Human) Forward Primer: TCCCTGGAGAAGAGCTACG (19 bp) Reverse Primer: GTAGTTTCGTGGATGCCACA (20 bp) Annealing Temperature: 60 °C 131 bp

• RTPrimerDB ID: 5

Gene Information HPRT1: hypoxanthine phosphoribosyltransferase 1 (Lesch-Nyhan syndrome) Alias Symbol(s): HPRT, HGPRT Organism: Homo sapiens (Hs, Human) (length: 94 bp) Genomic Amplicon: 4893bp Forward Primer: TGACACTGGCAAAACAATGCA (21 bp) Exon 6 Reverse Primer: GGTCCTTTTCACCAGCAAGCT (21 bp) Exon 6/7 Annealing Temperature: 60 °C

• RTPrimerDB ID: 8

Gene Information UBC: ubiquitin C Alias Symbol(s): HMG20 Organism: Homo sapiens (Hs, Human) (length: 133 bp) Forward Primer: ATTTGGGTCGCGGTTCTTG (19 bp) Reverse Primer: TGCCTTGACATTCTCGATGGT (21 bp) Annealing Temperature: 60 °C

• RTPrimerDB ID: 9

Gene Information YWHAZ: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Alias Symbol(s): KCIP-1 Organism: Homo sapiens (Hs, Human) (length: 94 bp) Forward Primer: ACTTTTGGTACATTGTGGCTTCAA (24 bp) Reverse Primer: CCGCCAGGACAAACCAGTAT (20 bp) Annealing Temperature: 60 °C

• RTPrimerDB ID: 4

Gene Information HMBS: hydroxymethylbilane synthase Alias Symbol(s): AIP, UPS, PBGD Organism: Homo sapiens (Hs, Human) This primer pair amplifies part of the following transcript: ENST00000278715 (Exons: 14, Transcript length: 1501 bp, Translation length: 361 residues) (length: 64 bp) Genomic amplicon:3252bp Forward Primer: GGCAATGCGGCTGCAA (16 bp) Exon 1 Reverse Primer: GGGTACCCACGCGAATCAC (19 bp) Exon 2 Annealing Temperature: 60 °C

• RTPrimerDB ID: 6

Gene Information RPL13A: ribosomal protein L13a Organism: Homo sapiens (Hs, Human) This primer pair amplifies part of the following transcript: ENST00000270634 (Exons: 8, Transcript length: 1125 bp, Translation length: 203 residues) (length: 126 bp) Forward Primer: CCTGGAGGAGAAGAGGAAAGAGA (23 bp) Reverse Primer: TTGAGGACCTCTGTGTATTTGTCAA (25 bp) Annealing Temperature: 60 °C

• RTPrimerDB ID: 7

Gene Information SDHA: succinate dehydrogenase complex, subunit A, flavoprotein (Fp) Alias Symbol(s): FP, SDH2, SDHF Organism: Homo sapiens (Hs, Human) This primer pair amplifies part of the following transcripts: ENST00000264932 (Exons: 15, Transcript length: 2280 bp, Translation length: 664 residues) (length: 86 bp) ENST00000327872 (Exons: 13, Transcript length: 1880 bp, Translation length: 517 residues) (length: 86 bp) Forward Primer: TGGGAACAAGAGGGCATCTG (20 bp) Reverse Primer: CCACCACTGCATCAAATTCATG (22 bp) Annealing Temperature: 60 °C

• RTPrimerDB ID: 2627

Gene Information TBP: TATA box binding protein Alias Symbol(s): GTF2D, SCA17, TFIID, GTF2D1 Organism: Homo sapiens (Hs, Human) This primer pair amplifies part of the following transcript: ENST00000230354 (Exons: 8, Transcript length: 1845 bp, Translation length: 339 residues) (length: 132 bp) Forward Primer: TGCACAGGAGCCAAGAGTGAA (21 bp) Reverse Primer: CACATCACAGCTCCCCACCA (20 bp) Annealing Temperature: 60 °C

• GAPDH

• B2M

• 28S

28S, from qpcrlist message 3097 <http://groups.yahoo.com/group/qpcrlistserver/message/3097> Multi species F tcatcagaccccagaaaagg R gattcggcaggtgagttgtt

• PPIB