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PCR inhibitors: Difference between revisions
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== Template inhibition == | == Template inhibition == | ||
The amount of template and the ratio of template to primer is an important parameter to optimise for difficult PCRs. Adding more template can in some cases decrease the amount of PCR product obtained. However, since inhibitors often enter the PCR reaction via the template preparation, decreasing template volume may improve PCR results indirectly by reducing the quantity of inhibiting contaminations. | |||
Typical starting amounts for genomic DNA template are 25-100 ng in a 25 µl PCR reaction. | |||
== See also == | == See also == | ||
