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PCR inhibitors: Difference between revisions
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[[Image:Inhibitor schematic.png|right|250px]] | [[Image:Inhibitor schematic.png|right|250px|thumb|Schematic of competitive inhibition of an enzyme. Note that active site inhibition is only one of many reasons why a PCR may not work.]] | ||
Many compounds also contained in the PCR template beside the target DNA can interfere with the polymerisation of nucleotides by the Taq or a related polymerase. This may result in false negative results, i.e. no PCR product even though the template is present. | Many compounds also contained in the PCR template beside the target DNA can interfere with the polymerisation of nucleotides by the Taq or a related polymerase. This may result in false negative results, i.e. no PCR product even though the template is present. | ||
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== See also == | == See also == | ||
* [[Polymerase chain reaction inhibitors|PCR inhibitor at the Wikipedia]] | |||
* [[Maheshri:PCR]] - has some tips on how to avoid inhibition | * [[Maheshri:PCR]] - has some tips on how to avoid inhibition | ||
