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PCR inhibitors: Difference between revisions
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→Inhibitors from the purification process
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== Inhibitors from the purification process == | == Inhibitors from the purification process == | ||
* alcohols like '''ethanol''' and '''2-propanol''' (isopropanol) from DNA template precipitation ([http://www.qiagen.com/literature/qiagennews/0497/974compl.pdf Qiagen test of sequencing inhibition by Welters et al. 1997]) | |||
* organic solvents like '''phenol''' from phenol/chloroform purification (PMID 8136148) | |||
* salts like '''KCl''', '''NaCl''' from precipitation | |||
* detergents like '''SDS''',.. from membrane lysis (PMID 2223070) | |||
== Inhibitors from the source tissue == | == Inhibitors from the source tissue == | ||
