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Griffin:Antibody Basics: Difference between revisions
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The 'F' stands for fragment and the 'ab' stands for antigen binding. This is the region where antigen binding takes place. The Fab portion of an antibody is made up of the N-terminal domains of both the heavy and light chains. This domain of the antibody is called the variable (V) domain (VH on the heavy chain, VL on the light chain) because there is such high variability between antibodies in this area. This allows recognition of thousands of different antigens. | The 'F' stands for fragment and the 'ab' stands for antigen binding. This is the region where antigen binding takes place. The Fab portion of an antibody is made up of the N-terminal domains of both the heavy and light chains. This domain of the antibody is called the variable (V) domain (VH on the heavy chain, VL on the light chain) because there is such high variability between antibodies in this area. This allows recognition of thousands of different antigens. | ||
===F(ab')2 fragment=== | |||
Pepsin cleaves a whole immunoglobulin to yield one F(ab')2 fragment and this is two antigen binding fragments linked by cysteine bonds. The 'prime ( ' )' is included in F(ab')2 since this fragment contains more amino acids than the F(ab) alone. | |||
F(ab')2 fragment secondary antibodies should be used when trying to avoid non-specific secondary antibody to Fc receptors on cell surfaces. | |||
==Primary antibody production== | ==Primary antibody production== | ||
