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Griffin:Antibody Basics: Difference between revisions
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→Carrier Protein
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*Carrier proteins provide a large number of functional moieties (amino, carboxyl, and sulfhydrul groups) that serve as "anchor points" for peptide conjugation. | *Carrier proteins provide a large number of functional moieties (amino, carboxyl, and sulfhydrul groups) that serve as "anchor points" for peptide conjugation. | ||
* | |||
====Carrier proteins==== | |||
*Bbovine serum albumin (BSA; 67kDa) | |||
*Keyhole limpet hemocyanin (KLH; 4.5e5-1.3e7 Da). | |||
*MBS: M-maleimidobenzoyl-N-hydroxysuccinimide ester Blue Carrier Protein | |||
Also ovalbumin (OVA), rabbit serum albumin (RSA), or bovine thyroglobulin (THY). | |||
This antigen carrier complex get subsequently mixed in with the Complete Freund's Adjuvant for a longer response time. | |||
===Adjuvant=== | ===Adjuvant=== | ||
