Griffin:Antibody Basics: Difference between revisions

Griffin:Antibody Basics (view source)

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 ===Peptide neutralization===
-The disappearance of staining in the presence of blocking peptide, i.e. preincubation of the antigen against which the antibody was raised with the antibody in theory should saturate antigen binding fragments and thereby no staining should be observed.
+[http://openwetware.org/wiki/Griffin:Antibody_Peptide_Neutralization Peptide Neutralization] is a negative control experiment that measures the disappearance of staining in the presence of blocking peptide (peptide antigen). Preincubation of the immunizing antigen with the purified antibody in theory should saturate antigen binding fragments and thereby no staining should be observed.
 ===Knockout animals===