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Griffin:Antibody Basics: Difference between revisions
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==Validation of Specificity== | ==Validation of Specificity== | ||
Validation primary antibody specificity is an ongoing topic of the utmost importance to scientists who choose to utilize immunoglobulin-based approaches. | Validation of primary antibody specificity is an ongoing topic of the utmost importance to scientists who choose to utilize immunoglobulin-based approaches. The mass of histology data, the often quite small n= number, combined with common lack of sufficient (molecular biological) controls are a compelling reason for reproducibility and disclosure of all applied compounds such as antibodies. However, the pure peptide sequence alone simply does not reveal or indicate specificity or reproducibility. Adequate control experiments are essential. | ||
===Direct ELISA=== | |||
===Peptide neutralization=== | ===Peptide neutralization=== | ||
