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Griffin:siRNA transfection: Difference between revisions
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→Cationic lipid-based transfection
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[[Image:cationliposome.jpg]] | [[Image:cationliposome.jpg]] | ||
Cationic lipid-based transfection reagents are suitable for transfecting into dividing cell cultures. Commercial examples include: [http://en.wikipedia.org/wiki/Lipofectamine Lipofectamine / L2000], [http://www.dharmacon.com Dharmafect], [http://www.molecula.com/ iFect], and [http://www.mirusbio.com/ TransIT TKO]. All of these reagents are proprietary formulations that | Cationic lipid-based transfection reagents are suitable for transfecting into dividing cell cultures. Commercial examples include: [http://en.wikipedia.org/wiki/Lipofectamine Lipofectamine / L2000], [http://www.dharmacon.com Dharmafect], [http://www.molecula.com/ iFect], and [http://www.mirusbio.com/ TransIT TKO]. | ||
All of these reagents are proprietary formulations that operate by forming lipsomal vesicles that house the siRNA payload and the slight + charge of the liposome is drawn to the cell membrane and bleb their way through the living cell membrane and into the cytoplasm. | |||
The efficiency of this process is cell type-dependent and must be determined in order to have confidence that the siRNA is getting into the cell. | |||
There are numerous commercial sources for transfection reagents for good reason; there are numerous cell types and lipsome structure will influence transfection efficiency in the multitude of experimental cell types that exist. | |||
In summary, the efficiency of a transfection reagent is a cell type-dependent event. Not all cells are receptive to transfection and for this reason, before any experiments are done, the researcher must determine transfection efficiency. | |||
==Verify transfection efficiency== | ==Verify transfection efficiency== | ||
