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Knight:Annealing and primer extension with Klenow polymerase: Difference between revisions
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Knight:Annealing and primer extension with Klenow polymerase (view source)
Revision as of 11:56, 26 May 2006
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This protocol uses annealing and primer extension to generate a short fragment of DNA (~100 bp). The DNA fragment is prepared for cloning by restriction digest. | This protocol uses annealing and primer extension to generate a short fragment of DNA (~100 bp). The DNA fragment is prepared for cloning by restriction digest. | ||
Also see [[Knight:Annealing and primer extension with Taq polymerase]] for generation for short DNA fragments for TA cloning. | |||
==Materials== | ==Materials== | ||
