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Kafatos:dsRNA Production by PCR: Difference between revisions
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forward primer (10 pmol/μl) 1 µl | forward primer (10 pmol/μl) 1 µl | ||
reverse primer (10 pmol/μl) 1 µl | reverse primer (10 pmol/μl) 1 µl | ||
ddH2O 40.3 μl | |||
'''Total: 50 μl''' | '''Total: 50 μl''' | ||
cDNA should be used as PCR template, but genomic DNA (at 1 μg/μl) is acceptable if the targeted region does not contain an intron and you are confident of the gene architecture.<BR> | cDNA should be used as PCR template, but genomic DNA (at 1 μg/μl) is acceptable if the targeted region does not contain an intron and you are confident of the gene architecture.<BR> | ||
=== Purification of PCR product === | === Purification of PCR product === | ||
