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TE: Difference between revisions
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*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions. | *Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions. | ||
*For dilalution of primers Water for Injection(water that is used for dialute injections)can be used effectively rather than going for TE and also for PCR as well. | *For dilalution of primers Water for Injection(water that is used for dialute injections)can be used effectively rather than going for TE and also for PCR as well. | ||
==See also== | |||
* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8018 10x TE buffer at Cold Spring Harbor Protocols] | |||
* [http://www.cytographica.com/lab/solutions/TE.htm 10x and 1x TE at cytographica] | |||
[[Category:Material]] and [[Category:Buffers]] | [[Category:Material]] and [[Category:Buffers]] | ||
