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TE: Difference between revisions
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10x TE recipe corrected
(→Notes) |
(10x TE recipe corrected) |
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TE buffer is often used to store DNA and RNA. The [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes. | TE buffer is often used to store DNA and RNA. The [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes. | ||
==Recipe== | ==Recipe 10x TE == | ||
{| | {| {{table}} | ||
|+ ''' | |+ '''for 1 liter of 10x TE stock solution''' | ||
|- | |- | ||
| | |volume || reagent || final conc. | ||
|- | |- | ||
| | |100 ml || 1M Tris-HCl pH 7.5 or 8.0 (see notes) || 100 mM | ||
|- | |- | ||
| | |20 ml || 0.5M EDTA pH 8.0 || 10 mM | ||
|- | |||
|880 ml || ddH<sub>2</sub>O || | |||
|} | |} | ||
→ 1x TE is 10 mM [[Tris]]-HCl and 1 mM [[EDTA]] | → 1x TE is 10 mM [[Tris]]-HCl and 1 mM [[EDTA]] | ||
