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Bacterial cell culture: Difference between revisions
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#Pipette the desired amount of [[Endy:Preparing Antibiotic Stocks|antibiotic]] into each tube along the wall. Do not put the non-sterile part of the pipette inside the tube and use a new tip for each tube. | #Pipette the desired amount of [[Endy:Preparing Antibiotic Stocks|antibiotic]] into each tube along the wall. Do not put the non-sterile part of the pipette inside the tube and use a new tip for each tube. | ||
#Vortex each tube for 1-2 seconds to mix well. | #Vortex each tube for 1-2 seconds to mix well. | ||
#Take the tubes to incubate. Turn the rotating rack off using the dial to decrease the speed. Do not use the switch because the motion is too abrupt. Add your tubes in a balanced | #Take the tubes to incubate. Turn the rotating rack off using the dial to decrease the speed. Do not use the switch because the motion is too abrupt. Add your tubes in a balanced layout. If you have an odd number, there are extra tubes for balance in the box on the floor next to the machine. Turn the rotating back on to 7. '''Do not forget to turn the rack back on.''' | ||
layout. If you have an odd number, there are extra tubes for balance in the box on the floor next to the machine. Turn the rotating back on to 7. '''Do not forget to turn the rack back on.''' | |||
#*Incubation once again is often at 37°C in the temperature controlled room. | #*Incubation once again is often at 37°C in the temperature controlled room. | ||
#Wait overnight or until your cells have reached the desired concentration. | #Wait overnight or until your cells have reached the desired concentration. | ||
#*The amount of time that one waits is dependent upon what you are growing the cells to do. To [[Miniprep|miniprep]] plasmid DNA, an overnight culture is sufficient. However, when doing measurements of protein levels, one should take care to take readings at the same cell culture density each time. | |||
