PMSF: Difference between revisions

PMSF (view source)

9 bytes removed , 1 September 2009

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 ==Purpose==
-PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purifications or coimunoprecipitations.
+PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purification or co-immunoprecipitation.
 Molecular W.: 174.19
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 ==Use==
-Don't try solution in water, it does not work. Take ethanol or methanol.
+PMSF is not soluble in water, dissolve in ethanol or methanol.
-mM stock solution are stable for months at 4°C.
+mM stock solution (0.035 g/mL) is stable for months at 4°C.
-A normal endconcentration of PMSF is probably about 1mM of course depending on the experimental setup.
+A common final concentration of PMSF is 1mM of course depending on the experimental setup.
 ==Safety==
-PMSF is poisenes and should be handeld carefully.
+PMSF is poisonous and should be handled carefully.
 [[Category:Material]] [[Category:Chemical]]