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HEPES: Difference between revisions
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A Good buffer <cite>Good66</cite><cite>Good74</cite><cite>Blanchard84</cite>. Stable pH vs. temperature, no amine groups, no metal chelation, near physiologic pH range. | A Good buffer <cite>Good66</cite><cite>Good74</cite><cite>Blanchard84</cite>. Stable pH vs. temperature, no amine groups, no metal chelation, near physiologic pH range. | ||
* pK<sub>a</sub> at 25C of 7.55 (a second | * pK<sub>a</sub> at 25C of 7.55 (7.31 at 37C) | ||
* a second pK<sub>a</sub> at pH 3 is not of interest | |||
* usable buffering range of 6.8 to 8.2 | * usable buffering range of 6.8 to 8.2 | ||
* molecular weight 238.3 | * molecular weight 238.3 | ||
