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Knight:Annealing and primer extension with Taq polymerase: Difference between revisions
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Knight:Annealing and primer extension with Taq polymerase (view source)
Revision as of 13:41, 5 August 2009
, 5 August 2009→Procedure
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Felix Moser (talk | contribs) |
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| Line 22: | Line 22: | ||
##94°C for 5 mins | ##94°C for 5 mins | ||
##94°C for 30 seconds | ##94°C for 30 seconds | ||
## | ##55°C for 30 seconds (or whatever an appropriate annealing temperature is) | ||
## | ##72°C for 30 seconds | ||
##Repeat steps 2-4 2-3 cycles | ##Repeat steps 2-4 2-3 cycles | ||
## | ##72°C for 5 mins | ||
#Use fresh 1μL PCR product in a [[Knight:TOPO TA cloning|TOPO TA cloning reaction]]. | #Use fresh 1μL PCR product in a [[Knight:TOPO TA cloning|TOPO TA cloning reaction]]. | ||
