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EGTA: Difference between revisions
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→EGTA 0.5M stock solution
(→EGTA 0.5M stock solution: pH adjustment) |
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| Line 9: | Line 9: | ||
* 19g EGTA (MW 380g/mol) | * 19g EGTA (MW 380g/mol) | ||
* ddH2O to 90ml | * ddH2O to 90ml | ||
* adjust pH 7.5/8.0 with solid NaOH ( | * adjust pH 7.5/8.0 with solid NaOH (>4g) | ||
* adjust volume to 100ml | * adjust volume to 100ml | ||
Note: EGTA will not go into solution without NaOH. Once the pH has been raised sufficiently it dissolves quickly. | Note: EGTA will not go into solution without NaOH. Once the pH has been raised sufficiently it dissolves quickly. For pH 7.5 the exact amount required is slightly above 4g. Add 3.5-4g immediately, then proceed carefully not to overshoot the desired pH. | ||
== Stability & storage == | == Stability & storage == | ||
