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EGTA: Difference between revisions
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→EGTA 0.5M stock solution: pH adjustment
(→EGTA 0.5M stock solution: pH adjustment) |
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| Line 9: | Line 9: | ||
* 19g EGTA (MW 380g/mol) | * 19g EGTA (MW 380g/mol) | ||
* ddH2O to 90ml | * ddH2O to 90ml | ||
* adjust pH 7.5/8.0 | * adjust pH 7.5/8.0 with solid NaOH (~4g) | ||
* adjust volume to 100ml | * adjust volume to 100ml | ||
Note: EGTA will not go into solution without NaOH. Once the pH has been raised sufficiently it dissolves quickly. The exactly amount required is slightly above 4g. Add 3.5-4g immediately, then proceed carefully not to overshoot the desired pH. | |||
== Stability & storage == | == Stability & storage == | ||
