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BISC110: Series 4 Lab 11 Enzymology: Difference between revisions
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→Series 4- Experiment in Gene Regulation & Enzymology
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GENE REGULATION — ENZYME ASSAY & TRANSFORMATION | '''GENE REGULATION — ENZYME ASSAY & TRANSFORMATION''' | ||
The final series of experiments in BISC 110 will concentrate on the relationship between genes and proteins. In lab today, you will be using two organisms, one eukaryote and one prokaryote, to demonstrate two ways that protein production is regulated at the genetic level within these cells. You will also see how this knowledge is used to create genetically engineered bacteria capable of producing large amounts of a target protein. | The final series of experiments in BISC 110 will concentrate on the relationship between genes and proteins. In lab today, you will be using two organisms, one eukaryote and one prokaryote, to demonstrate two ways that protein production is regulated at the genetic level within these cells. You will also see how this knowledge is used to create genetically engineered bacteria capable of producing large amounts of a target protein. | ||
A. GENETIC REGULATION OF ENZYME PRODUCTION IN YEAST BY GLUCOSE REPRESSSION. | '''A. GENETIC REGULATION OF ENZYME PRODUCTION IN YEAST BY GLUCOSE REPRESSSION.''' | ||
Yeasts are eukaryotic organisms that have been used for centuries to ferment beverages and to leaven bread. However, the usefulness of yeast extends outside of the kitchen. Yeast can be used as powerful tools for genetic research because they have a short generation time, well-defined metabolic pathways, and many interesting mutants. | Yeasts are eukaryotic organisms that have been used for centuries to ferment beverages and to leaven bread. However, the usefulness of yeast extends outside of the kitchen. Yeast can be used as powerful tools for genetic research because they have a short generation time, well-defined metabolic pathways, and many interesting mutants. | ||
