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**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%. | **Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%. | ||
Another buffer used for washing in immunohistology is PBS Triton, which contains 0.1%-0.3% of the detergent Triton X-100. A 1% Triton X-100 can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment. | |||
==Other Links== | ==Other Links== | ||
