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Bacterial cell culture: Difference between revisions
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For a typical liquid culture, use 5 ml of appropriate medium. The amount in each tube does not have to be exact if you are just trying to culture cells for their precious DNA. | For a typical liquid culture, use 5 ml of appropriate medium. The amount in each tube does not have to be exact if you are just trying to culture cells for their precious DNA. | ||
#Streak an [[ | #Streak an [[Endy:Pouring plates|agar plate]] from glyerol stock. Incubate plates until colonies grow. | ||
#*Most incubations with ''E. coli'' take place at 37°C. Often bacteria with temperature sensitive mutations need to be grown at 30° instead. | #*Most incubations with ''E. coli'' take place at 37°C. Often bacteria with temperature sensitive mutations need to be grown at 30° instead. | ||
#*The reason you need to streak plates is to be certain that you are starting from a single clonal population of cells. In this case, colonies that are picked are assumed to arise from a single cell dividing to form the colony. | #*The reason you need to streak plates is to be certain that you are starting from a single clonal population of cells. In this case, colonies that are picked are assumed to arise from a single cell dividing to form the colony. | ||
