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Griffin:Antibody Basics: Difference between revisions
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7. Re-equilibrate the column in PBS with sodium azide. | 7. Re-equilibrate the column in PBS with sodium azide. | ||
===Polyethylene Glycol Precipitation=== | |||
Hybridoma supernatants precipitation with polyethylene glycol 6000 (PEG 6000) and finally reprecipitated using an ammonium sulphate procedure. The PEG 6000 treatment causes a density change in the ammonium sulphate immunoglobulin precipitate, and resulted in the formation of a pellicle which contained pure mouse monoclonal antibody. The protocol removed contaminating bovine serum immunoglobulin as well as other serum and cellular protein from the monoclonal antibody preparations. | |||
<biblio> | |||
#Paper1 pmid=1401962 | |||
</biblio> | |||
==Ascites== | ==Ascites== | ||
