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Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins: Difference between revisions
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Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins (view source)
Revision as of 18:54, 14 June 2008
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##Deionized Water 950 ml | ##Deionized Water 950 ml | ||
##Total Volume 1000 ml | ##Total Volume 1000 ml | ||
*Mix thoroughly and set aside 800 ml of the 1X NuPAGE® SDS Running Buffer for use in the lower (Outer) Buffer Chamber of the XCell SureLock Mini-Cell | |||
*Immediately, prior to electrophoresis, add 500μ l of NuPAGE® Antioxidant to 200 ml of 1X NuPAGE® SDS Running for use in the Upper (Inner) Buffer Chamber of the XCell SureLock. Mini-Cell. Mix thoroughly. | |||
#Remove the NuPAGE® Gel from the pouch | #Remove the NuPAGE® Gel from the pouch | ||
#Rinse the gel cassette with deionized water. Peel off the tape from the bottom of the cassette. | #Rinse the gel cassette with deionized water. Peel off the tape from the bottom of the cassette. | ||
