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##Deionized Water 950 ml
##Total Volume 1000 ml
###*Mix thoroughly and set aside 800 ml of the 1X NuPAGE® SDS Running Buffer for use in the lower (Outer) Buffer Chamber of the XCell SureLock Mini-Cell###*Immediately, prior to electrophoresis, add 500μ l of NuPAGE® Antioxidant to 200 ml of 1X NuPAGE® SDS Running for use in the Upper (Inner) Buffer Chamber of the XCell SureLock. Mini-Cell. Mix thoroughly.
#Remove the NuPAGE® Gel from the pouch
#Rinse the gel cassette with deionized water. Peel off the tape from the bottom of the cassette.
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