User contributions for Ashley Chou

21 July 2008

• 13:3413:34, 21 July 2008 diff hist +715 Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins No edit summary

17 July 2008

• 18:0418:04, 17 July 2008 diff hist 0 Jacobs:Back Door →Research Protocols
• 18:0418:04, 17 July 2008 diff hist +63 Jacobs:Back Door →Research Protocols
• 18:0218:02, 17 July 2008 diff hist 0 Jacobs:Protocol OFF (Large Chambers) No edit summary current
• 18:0118:01, 17 July 2008 diff hist +7,952 N Jacobs:Protocol OFF (Large Chambers) New page: ==Materials== *Cells at ~80-90% confluence (plate ~250K cells/slide 2 days prior) *Tweezers *-MEM / 5% FBS / 5% CS *Pipette Bulbs *Tubing (1/8 ID x ¼ OD x 1/16 wall) *Cell Scraper *...

16 July 2008

• 14:0514:05, 16 July 2008 diff hist −195 Jacobs:Back Door →Useful Links

15 July 2008

• 17:4317:43, 15 July 2008 diff hist +103 N Jacobs:Contact New page: To reach any of the lab members, see their contact information under Lab Members
• 16:3216:32, 15 July 2008 diff hist +445 Jacobs:Publications No edit summary
• 16:2916:29, 15 July 2008 diff hist +37 Jacobs:Publications No edit summary
• 16:2916:29, 15 July 2008 diff hist +4 Jacobs:Publications No edit summary
• 16:2816:28, 15 July 2008 diff hist +8 Jacobs:Publications No edit summary
• 16:2816:28, 15 July 2008 diff hist +5,371 N Jacobs:Publications New page: Malone, A.M.D., Anderson, C.T., Tummala, P., Kwon, R.Y., Johnston, T.R., Jacobs, C.R., Primary Cilia Mediate Mechanosensing in Bone Cells by a Calcium-Independent Mechanism. Proceedings of...
• 16:2716:27, 15 July 2008 diff hist +155 Jacobs:Research →Active Projects
• 16:2716:27, 15 July 2008 diff hist +143 Jacobs:Research →Active Projects
• 16:2616:26, 15 July 2008 diff hist +3,063 N Jacobs:Research:Cellular Basis for Optimal Osteogenic Physical Interventions New page: '''Objective''' Disuse bone loss is a critical problem in rehabilitation that has very little progress made in terms of effective countermeasures. It is a consequence of the well accepted...
• 16:2516:25, 15 July 2008 diff hist +3,063 N Jabos:Research:Cellular Basis for Optimal Osteogenic Physical Interventions New page: '''Objective''' Disuse bone loss is a critical problem in rehabilitation that has very little progress made in terms of effective countermeasures. It is a consequence of the well accepted... current
• 16:2416:24, 15 July 2008 diff hist +193 Jacobs:Research →Active Projects
• 16:2316:23, 15 July 2008 diff hist +1,811 N Jacobs:Research:Structural Analysis of Cytoskeletal Deformation in Bone Cells Exposed to Shear Stress New page: '''Objective''' Understanding the micromechanics of cellular and cytoskeletal deformation may be key in understanding the process of mechanotransduction. Many questions regarding the mech...
• 16:2116:21, 15 July 2008 diff hist +149 Jacobs:Research →Active Projects
• 16:2116:21, 15 July 2008 diff hist −2 Jacobs:Research:Microfabrication of 3D Microfluidic Environments for Osteocytes No edit summary
• 16:2016:20, 15 July 2008 diff hist +1,252 N Jacobs:Research:Microfabrication of 3D Microfluidic Environments for Osteocytes New page: '''Objective''' Determine whether oscillating fluid flow applied to the osteocyte process alone is sufficient to elicit the response observed in parallel plate monolayers. Completion of t...
• 16:1816:18, 15 July 2008 diff hist +117 Jacobs:Research →Active Projects
• 16:1716:17, 15 July 2008 diff hist +1,688 N Jacobs:Research:Loading Induced Fluid Flow and Disuse Bone Loss New page: '''Objective''' Microgravity conditions result in a dramatic reduction of normal skeletal loading due to decreased weight bearing leading to rapid bone loss. A variety of countermeasures ...
• 16:1616:16, 15 July 2008 diff hist +131 Jacobs:Research →Active Projects
• 16:1416:14, 15 July 2008 diff hist +3,076 N Jacobs:Research:Mechanotransduction in Bone via Oscillatory Fluid Flow New page: '''Objective''' Bone cells occupy fluid filled voids (lacunae) in the mineralized matrix and interconnected by small tubes (canaliculi). As the bone matrix is cyclically loaded, fluid fl...
• 16:1116:11, 15 July 2008 diff hist +46 Jacobs:Lab Members →Current (Stanford)
• 16:1016:10, 15 July 2008 diff hist −2 Jacobs:Lab Members →Current (Columbia)
• 16:0916:09, 15 July 2008 diff hist +18 Jacobs:Lab Members →Current (Columbia)
• 15:5715:57, 15 July 2008 diff hist +56 Jacobs:Meeting Schedule No edit summary

14 July 2008

• 00:1600:16, 14 July 2008 diff hist +793 Jacobs:Meeting Schedule No edit summary

11 July 2008

• 20:0420:04, 11 July 2008 diff hist −729 Jacobs:Meeting Schedule Removing all content from page
• 20:0220:02, 11 July 2008 diff hist −58 Jacobs:Meeting Schedule No edit summary
• 19:5819:58, 11 July 2008 diff hist −27 Jacobs:Meeting Schedule →Google Lab calendar
• 19:5819:58, 11 July 2008 diff hist +582 Jacobs:Meeting Schedule No edit summary
• 19:4319:43, 11 July 2008 diff hist +10 Jacobs:Meeting Schedule No edit summary
• 19:4319:43, 11 July 2008 diff hist +222 N Jacobs:Meeting Schedule New page: '''Thursday, June 17th (9:00 a.m.): Alesha''' '''Friday, June 25th (9:00 a.m.): Sara''' '''Monday, August 11th (TBD): Ron''' '''Thursday, August 21st (9:00 a.m.): Julie''' '''Thursday,...

10 July 2008

• 14:3414:34, 10 July 2008 diff hist −32 Jacobs:Back Door →Research Protocols
• 14:3314:33, 10 July 2008 diff hist +26 Jacobs:Back Door →Research Protocols
• 14:2914:29, 10 July 2008 diff hist 0 Jacobs:Back Door →Research Protocols
• 14:2814:28, 10 July 2008 diff hist +81 Jacobs:Back Door →Research Protocols
• 14:2514:25, 10 July 2008 diff hist +2,550 N Jacobs:Protocol Freezing and Thawing Cells New page: ==Procedure== '''Freezing''' # Check cell culture log and be sure there is room for frozen vials # Label vials with cell type, date, passage number, etc. # Collect cell suspension by t...
• 13:4613:46, 10 July 2008 diff hist +195 Jacobs:Back Door →Useful Links

7 July 2008

• 14:3314:33, 7 July 2008 diff hist +45 Jacobs:Back Door →Research Protocols
• 14:3114:31, 7 July 2008 diff hist +7,628 N Jacobs:Protocol Loading Mice New page: ==Procedure== '''Loading (Days 1-3)''' #Turn on Enduratec controllers then turn on computer (pswd: enduratec; Hyperkernel enabled) #Set up the weigh station #Assemble anesthesia device ... current

17 June 2008

• 17:1117:11, 17 June 2008 diff hist +367 Jacobs:Back Door →Research Protocols
• 17:1117:11, 17 June 2008 diff hist −368 Jacobs:Back Door →Teaching Protocols
• 02:3702:37, 17 June 2008 diff hist +97 Jacobs:Back Door →Research Protocols
• 02:3702:37, 17 June 2008 diff hist +1 Jacobs:Protocol Total RNA Isolation using Tri Reagent No edit summary current
• 02:3602:36, 17 June 2008 diff hist +3,208 N Jacobs:Protocol Total RNA Isolation using Tri Reagent New page: ==Materials== * Tri-Reagent (Sigma T9424) * Cell scraper * Pipetman * Pipet tips * Autoclaved microcentrifuge tube * Chloroform (Sigma (C2432) * Centrifuge * Isopropanol (Sigma I9516) * 75...
• 02:3002:30, 17 June 2008 diff hist −742 Jacobs:Protocol Subculturing 3T3 Fibroblasts No edit summary current