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PBS: Difference between revisions
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==Purpose== | |||
PBS stands for phosphate buffered saline and is often used because it is isotonic to cells. | PBS stands for phosphate buffered saline and is often used because it is isotonic to cells. | ||
==Recipe== | |||
1x PBS is 137 mM NaCl, 10 mM Phosphate, 2.7 mM KCl, pH 7.4 | 1x PBS is 137 mM NaCl, 10 mM Phosphate, 2.7 mM KCl, pH 7.4 | ||
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#* 80 g NaCl | #* 80 g NaCl | ||
#* 2 g KCl | #* 2 g KCl | ||
#* 14.4 g | #* 14.4 g Na<sub>2</sub>HPO<sub>4</sub> | ||
#* 2.4 g | #* 2.4 g KH<sub>2</sub>PO<sub>4</sub> | ||
#* 800 mL distilled | #* 800 mL distilled H<sub>2</sub>O | ||
# Adjust pH to 7.4 with HCl | # Adjust pH to 7.4 with HCl | ||
# Add | # Add H<sub>2</sub>O to 1L | ||
# Autoclave for 20 minutes on liquid cycle. Store at RT. | # Autoclave for 20 minutes on liquid cycle. Store at RT. | ||
