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DNA extraction - Salting Out: Difference between revisions
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m (DNA extraction: Salting Out moved to DNA extraction - Salting Out: Mistakenly put in a colon seperator, correcting early) |
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#Incubator/Water Bath: preferably shaking | #Incubator/Water Bath: preferably shaking | ||
#Centrifuge: preferably refrigerated | #Centrifuge: preferably refrigerated | ||
#Vortex | |||
==Procedure== | ==Procedure== | ||
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#Homogenise (or simply place) tissue in solution | #Homogenise (or simply place) tissue in solution | ||
#Incubate at 55°C for 1 hour to overnight | #Incubate at 55°C for 1 hour to overnight | ||
# | #Mix by vortexing then centrifuge at maximum speed in a benchtop centrifuge for 2 minutes | ||
#Transfer supernatant into a new tube | #Transfer supernatant into a new tube | ||
#Add 1/10 volume of Sodium Acetate 3M (pH5.2) | #Add 1/10 volume of Sodium Acetate 3M (pH5.2) | ||
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==Acknowledgments== | ==Acknowledgments== | ||
*Damien Broderick from Queensland Depaqrtment of Primary Industries and Fisheries supplied | *Damien Broderick from Queensland Depaqrtment of Primary Industries and Fisheries supplied the foundation to this protocol. | ||
==References== | ==References== | ||
