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DNA extraction - Salting Out: Difference between revisions
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#Centrifuge (preferably at 4°C) at maximum speed in a benchtop centrifuge for 20 minutes | #Centrifuge (preferably at 4°C) at maximum speed in a benchtop centrifuge for 20 minutes | ||
#Wash pellet with 70% ethanol, dry and resuspend in water or TE | #Wash pellet with 70% ethanol, dry and resuspend in water or TE | ||
==Critical steps== | ==Critical steps== | ||
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==Troubleshooting== | ==Troubleshooting== | ||
You may wish to kill the Proteinase K (95°C, 10 minutes) | |||
==Notes== | ==Notes== | ||
* | *I routinely rely on the denaturation step of Hot-start Taq in my PCR to take care killing proteinase K (doesn't seem to effect TAq in this case) | ||
*Successfully used on fish, mammal and insect tissue. | *Successfully used on fish, mammal and insect tissue. | ||
*Works well in 96 well plate format, to remove ethanol following precipitation/wash simply invert the plate over a sink then spin gently (~100 rcf) inverted over absorbant paper. | *Works well in 96 well plate format, to remove ethanol following precipitation/wash simply invert the plate over a sink then spin gently (~100 rcf) inverted over absorbant paper. | ||
